Purification and Properties of (1.3)- -Glucanases from Bacillus circulans WL-12

Expression and characterization of the chitin-binding domain of chitinase A1 from Bacillus circulans WL-12.

Chitinase A1 from Bacillus circulans WL-12 comprises an N-terminal catalytic domain, two fibronectin type III-like domains, and a C-terminal chitin-binding domain (ChBD). In order to study the biochemical properties and structure of the ChBD, ChBD(ChiA1) was produced in Escherichia coli using a pET expression system and purified by chitin affinity column chromatography. Purified ChBD(ChiA1) spe...

Chitinase system of Bacillus circulans WL-12 and importance of chitinase A1 in chitin degradation.

Bacillus circulans WL-12, isolated as a yeast cell wall-lytic bacterium, secretes a variety of polysaccharide-degrading enzymes into culture medium. When chitinases of the bacterium were induced with chitin, six distinct chitinase molecules were detected in the culture supernatant. These chitinases (A1, A2, B1, B2, C, and D) showed the following distinct sizes and isoelectric points: Mr 74,000,...

Nucleotide sequence of a 1,3-1,4-beta-glucanase-encoding gene in Bacillus circulans WL-12.

NoteThe Action of Bacinus circulans WL - 12 Chitinaseson Partially N - Acetylated Chitosan

Both chitinase Al and D from Bacitlas eireulans WL-12 speeifically hydrolyzed the IY-acetyl-fi-D-glucosaminidic bonds in 50% ?VLacetylated chitesan molecules to produce hetero-oligosaccharides with GlcNAc at the reducing end residues, together with GlcNAc and (GlcNAc}2. ClcN-GlcNAc and ClcN-GlcNAc-GlcNAc were produced as major hydrolysis products with chitinase Al and D, respectiyely, but GlcN-...

Gene cloning of chitinase A1 from Bacillus circulans WL-12 revealed its evolutionary relationship to Serratia chitinase and to the type III homology units of fibronectin.

A chitinase gene of Bacillus circulans WL-12 was cloned into Escherichia coli by transforming HB101 cells with a recombinant plasmid composed of chromosomal DNA fragments prepared from B. circulans WL-12 and the plasmid vector pKK223-3. DNA sequencing analysis revealed that the region necessary for the normal expression of chitinase activity contained one open reading frame of 2097 base pairs w...